In particular, how does gatipuzumab interact with the MUC1 antigen in cancerous cells?

Targeted by gatipuzumab, the MUC1 antigen's variable region is overexpressed in a large number of epithelial malignancies. It attaches itself to the protein in its hypoglycosylated form, facilitating efficient identification and remedial action.

In which types of cancers has Gatipotuzumab shown the most efficacy in clinical trials?

In clinical trials, gemcitabine has demonstrated exceptional efficacy against non-small cell lung cancer, ovarian cancer, and breast cancer. Tumor shrinkage and higher survival rates are evidence of its effectiveness.

Is it possible to combine gatipotuzumab with other forms of treatment?

Indeed, in order to improve therapeutic results, intratuzumab is frequently used in conjunction with radiation, chemotherapy, or other monoclonal antibodies. Patient response rates have improved with such combo medicines.

Anti-Human MUC1 Recombinant Antibody (TAB-026ML)

CAT#: TAB-026ML

Recombinant monoclonal antibody to MUC1. It is a human monoclonal antibody that can be potentially used in the treatment of Solid tumors.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human MUC1 Recombinant Antibody (TAB-026ML) in SDS-PAGE

SDS-PAGE analysis of TAB-026ML in reduced (Lane 1) and non-reduced (Lane 2) conditions.

SEC-HPLC

Figure 2 Anti-Human MUC1 Recombinant Antibody (TAB-026ML) in HPLC

The purity of TAB-026ML was greater than 95% as determined by SEC-HPLC.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg

Normal Tissue Figure 5 Stomach

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg

Normal Tissue Figure 8 Testis

Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg

RNA Expression Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized

Type
IgG1, κ

Specificity
Human MUC1

Species Reactivity
Human

Applications
ELISA, IHC, FC, IP, IF, FuncS, Stim

Conjugate
Unconjugated

Related Disease
Solid tumors

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The MUC1 antibody has been reported in applications of ELISA, IHC, FC, IP, IF, FuncS, Stim.

Target

Alternative Names
Mucin 1; polymorphic epithelial mucin; PEM; episialin; CD227

Gene ID
4582

UniProt ID
P15941

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

Reliable in ELISA

We have been using Gatipotuzumab for ELISA, and it has provided consistent and reliable results. The optimal concentration was easy to determine, and the sensitivity is outstanding.

High specificity in FC

Flow cytometry with Gatipotuzumab has been very efficient. The antibody labeled MUC1-expressing cancer cells with high specificity, which facilitated our analysis significantly.

Minimal cross-reactivity

We noticed minimal cross-reactivity with non-human MUC1, which was expected but good to confirm. It's highly specific to human MUC1, as advertised.

Q&As

In particular, how does gatipuzumab interact with the MUC1 antigen in cancerous cells?

A: Targeted by gatipuzumab, the MUC1 antigen's variable region is overexpressed in a large number of epithelial malignancies. It attaches itself to the protein in its hypoglycosylated form, facilitating efficient identification and remedial action.
In which types of cancers has Gatipotuzumab shown the most efficacy in clinical trials?

A: In clinical trials, gemcitabine has demonstrated exceptional efficacy against non-small cell lung cancer, ovarian cancer, and breast cancer. Tumor shrinkage and higher survival rates are evidence of its effectiveness.
Is it possible to combine gatipotuzumab with other forms of treatment?

A: Indeed, in order to improve therapeutic results, intratuzumab is frequently used in conjunction with radiation, chemotherapy, or other monoclonal antibodies. Patient response rates have improved with such combo medicines.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-026ML, RRID: AB_3111759)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-026ML.pdf

MSDS

MSDS - TAB-026ML.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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HPAB-1159WJ-S(P)	Mouse Anti-MUC1 Recombinant Antibody (clone 12E); scFv Fragment	ELISA, ICC	Mouse scFv
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TAB-0278CL	Human Anti-MUC1 Recombinant Antibody (TAB-0278CL)	ELISA, Internalization, BI, FuncS	Human IgG
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TAB-433MZ-F(E)	Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-433MZ-F(E))	ELISA, FC	Human Fab
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TAB-412MZ-F(E)	Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-412MZ-F(E))	ELISA	Chimeric (mouse/human) Fab

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TAB-411MZ-F(E)	Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-411MZ-F(E))	ELISA	Mouse Fab
TAB-416MZ-F(E)	Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-416MZ-F(E))	IF	Mouse Fab
TAB-417MZ-F(E)	Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-417MZ-F(E))	IF	Mouse Fab
TAB-418MZ-F(E)	Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-418MZ-F(E))	ELISA, SDS-PAGE, IHC	Mouse Fab
TAB-419MZ-F(E)	Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-419MZ-F(E))	ELISA, SDS-PAGE, IHC	Mouse Fab

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Gly-012LC	Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated)	ELISA, FC, IHC	Humanized antibody
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Gly-012LC-1	Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated)	ELISA, FC, IHC	Humanized antibody

CAT	Product Name	Application	Type
Gly-109LC	Recombinant Anti-Human MUC1 Antibody (Fc glycosylation)	ELISA	Human antibody

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Gly-121LC	Recombinant Anti-Human MUC1 Antibody (Non-glycosylated)	ELISA	Mouse antibody
Gly-122LC	Recombinant Anti-Human MUC1 Antibody (Non-glycosylated)	ELISA	Mouse antibody
Gly-124LC	Recombinant Anti-Human MUC1 Antibody (Non-glycosylated)	ELISA	Mouse antibody

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BRD-0379MZ	Chicken Anti-MUC1 Polyclonal IgY	IHC, WB	Chicken antibody

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MHC-LC096	PE-A*02:01/Human MUC1 (LLLLTVLTV) MHC Tetramer	FCM
MHC-LC097	APC-A*02:01/Human MUC1 (LLLLTVLTV) MHC Tetramer	FCM
MHC-LC781	A*0201/Human MUC-1 (LLLLTVLTV) MHC Tetramer	FCM
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MOR-2320	Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB)	IHC-P, IHC-Fr	IgG

CAT	Product Name	Application	Type
EPAF-0838LC	Recombinant Mouse Anti-Human MUC1 Antibody (SM3)	ELISA	IgG1
EPAF-0581CQ	Recombinant Mouse Anti-Human MUC1 Antibody (B27.29)	Neut, FC	IgG1
EPAF-0583CQ	Recombinant Mouse Anti-Human MUC1 Antibody (BC4E549)	Neut, FC	IgG1
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FAMAB-0225-YC-F(E)	Mouse Anti-MUC1 Recombinant Antibody (clone MUSE11); Fab Fragment	IHC	Mouse Fab
HPAB-0272CQ-F(E)	Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0272CQ-F(E))	ELISA, FC, FuncS	Human Fab
PFBC-400	Mouse Anti-MUC1 Recombinant Antibody (clone SM3); Fab Fragment	FC, IHC-Fr, IF, ELISA, IHC-P	Mouse Fab
HPAB-0207-YJ-F(E)	Human Anti-MUC1 Recombinant Antibody (clone Clivatuzumab (hPAM4)); Fab Fragment	ELISA, IHC, FuncS	Humanized Fab
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HPAB-0670-CN	Human Anti-MUC1 Recombinant Antibody (HPAB-0670-CN)	FC	Human IgG4, κ
HPAB-0272CQ	Human Anti-MUC1 Recombinant Antibody (HPAB-0272CQ)	ELISA, FC, FuncS	Human IgG1, κ
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AFC-TAB-H56	Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H56)	FuncS, IF, Neut, ELISA, FC	ADCC enhanced antibody
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AFC-TAB-026ML	Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-026ML)	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-166	Afuco™ Anti-MUC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-166)	IP, IF, FuncS, FC, Neut, ELISA	ADCC enhanced antibody
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HPAB-AP745-YC	Mouse Anti-MUC1 Recombinant Antibody (HPAB-AP745-YC)	ELISA	Mouse IgM

CAT	Product Name	Application	Type
VS-0424-XY192	AbPlus™ Anti-MUC1 Magnetic Beads (139H2)	IP, Protein Purification
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CAT	Product Name	Application	Type
VS-0125-FY26	Human Anti-MUC1 (clone 1B2) scFv-Fc Chimera	FC	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY169	CytoStream™ Mouse Anti-MUC1 Recombinant Antibody (VS-0225-XY169)	FC	Mouse IgG1, kappa

CAT	Product Name	Application
VS-0325-XY1394	Anti-MUC1 Immunohistochemistry Kit	IHC
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VS-0525-XY4576	Anti-Mouse MUC1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-YC379	Recombinant Anti-MUC1 Vesicular Antibody, EV Displayed (VS-0425-YC379)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC131	Recombinant Anti-MUC1 Biparatopic Antibody, Tandem scFv	ELISA, IHC	Tandem scFv

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